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. Author manuscript; available in PMC: 2014 Oct 1.
Published in final edited form as: Curr Opin Biotechnol. 2013 Jun 13;24(5):916–925. doi: 10.1016/j.copbio.2013.05.005

Figure 2. Remodeling of TEV after implantation.

Figure 2

Fibrin-based TEV were prepared from vascular smooth muscle cells (V-TEV; A, C) that were isolated from saphenous veins or from BM-MSC derived SMC (BM-TEV; B, D) and implanted into the external jugular veins of 12-week old lambs. At the indicated times (15 or 5 weeks) the tissues were explanted and processed for histology. (A, B) Mason’s trichrome showed abundant collagen (blue/green). (C, D) Verhoff’s stain showed elastin fibers as black lines. Note the abundant expression and fibrillar organization of elastin (black lines) only in BM-TEV but not in V-TEV despite the longer implantation time of the later. Luminal surface is at the top of each panel and blood flows in the direction that crosses the plane of the page. (E, F) Quantitative RT-PCR for collagen (E) and elastin (F) expression in neonatal (n1–n3) BM-MSC, adult (a1–a3) BM-MSC or V-SMC. The data was normalized relative to GAPDH. The relative expression level was defined as the ratio of the normalized expression level of each gene in BM-SMC to that in V-SMC from umbilical veins of neonatal lambs. Note the difference in elastin expression between nBM-MSC, aBM-MSC and V-SMC.