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. Author manuscript; available in PMC: 2014 Dec 31.
Published in final edited form as: Mol Immunol. 2013 Aug 1;56(4):423–433. doi: 10.1016/j.molimm.2013.06.008

Figure 2. Wnt signaling suppresses CTL effector functions.

Figure 2

Sorted OT1 cells were stimulated with antigen+B7+IL-12 in the presence of TWS119 at different concentrations. Programmed CTLs were harvested at 72 hrs. (A) The expression of IFNγ and granzyme B (GZB). (B) The expression of CD62L, PD1 and Annexin V. (C) Transcriptional regulation of CD62L by TWS119. OT1 cells were stimulated for 3 days under 3 SI in the presence or absence of TWS119 at 2.5 µM, and then were harvested for real-time PCR examination. CD62L and housekeeping gene GAPDH were analyzed in triplicate in real-time RT-PCR assays. Relative mRNA amounts were normalized with respect to expression levels in Naïve OT1 control (fold change=1). The results are expressed as mean + SD of three independent experiments. Asterisks indicate statistical significance. *, P < 0.05; **, P < 0.01; ***, P < 0.001. ns: not significant. These will be followed in the rest of paper. These are representatives of three independent experiments with similar results.