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Cis-regulatory analysis to uncover the regulatory inputs driving early sip1 expression. A) Diagram of the 16 kb region upstream of sip1 and reporter constructs shown earlier to have cis-regulatory activity. All constructs include a GFP reporter and sequence tag for expression analysis (Nam et al., 2010). B) Identification of active modules driving sip1 expression through a series of deletion constructs. C) Mutation of otx-binding sites, resulting in reduced expression level.
