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. Author manuscript; available in PMC: 2014 Sep 20.
Published in final edited form as: ACS Chem Biol. 2013 Jul 10;8(9):1907–1911. doi: 10.1021/cb400340k

Figure 4.

Figure 4

Trypsin-catalyzed hydrolysis of 50 µM HD5red in the absence and presence of 1.2 equiv of Zn(II). (a) Analytical HPLC traces (220 nm). Left: no Zn(II) added; right: addition of Zn(II). The reactions were quenched at t = 1, 2, 5, 15, 30, and 60 min (units not shown). (b) Integrated HD5red peak area versus time. These data correspond to the peaks exhibited in Panel A. Full HPLC chromatograms and HD5-TE controls are presented in Figure S7.