Fig. 6.
Cultured cholangiocytes produce interleukin (IL)-6 and transforming growth factor-β (TGF-β) proteins. Cholangiocytes from the cell line were cultured alone (white bar) or with interferon-γ (IFN-γ) (black bar), poly I:C (grey bar) or Rhesus rotavirus (hatched bar) and culture supernatants were collected at 24, 48 and 72 h to assess for IL-6 (A) and TGF-β (B) proteins by enzyme-linked immunosorbent assay. Values represent the mean pg/ml ± SEM. Cholangiocytes treated with poly I:C generated modest amounts of IL-6 and those treated with IFN-γ produced abundant amounts of IL-6 and TGF-β compared with unstimulated controls at 24 and 48 h in culture.