Table 3.
Analyses of Itpr3 WT and KO mice two-bottle choice test preference scores
| Two-way ANOVA |
||||||
|---|---|---|---|---|---|---|
| Compound | Cohort and Order | Group Composition | Group | Concentration | Group × Concentration | Concentrations Supporting Strain Differences |
| Saccharin | 5b | 9 WT, 8 KO (M) | F(1,15) = 23.5, P = 0.0002 | F(6,90) = 5.09, P = 0.0002 | F(6,90) = 2.97, P = 0.0108 | 1, 3.2, 10, 32 mM |
| Sucrose | 1b | 11 WT, 11 KO (F) | F(1,20) = 15.7, P = 0.0007 | F(7,140) = 45.9, P < 0.0001 | F(7,140) = 3.50, P = 0.0017 | 1, 2, 4% |
| Maltose | 4b | 8 WT, 8 KO (F) | F(1,14) = 23.8, P = 0.0002 | F(7,98) = 29.2, P < 0.0001 | F(7,98) = 3.92, P = 0.0008 | 1, 2, 4, 8% |
| Polycose | 3b | 9 WT, 9 KO (M) | F(1,16) = 16.6, P = 0.0008 | F(7,112) = 27.6, P < 0.0001 | F(7,112) = 4.66, P = 0.0001 | 0.5, 1, 2, 4, 20% |
| MSG | 6a, 8a | 18 WT, 16 KO (F) | F(1,32) = 2.33, P = 0.1368 | F(6,192) = 32.70, P < 0.0001 | F(6,192) = 1.75, P = 0.1117 | 10, 32 mM* |
| IMP | 6b | 9 WT, 8 KO (F) | F(1,15) = 2.69, P = 0.1214 | F(8,120) = 16.4, P < 0.0001 | F(8,120) = 3.10, P = 0.0033 | 10, 32 mM |
| Na3HP2O7 | 7a | 8 WT, 8 KO (M) | F(1,14) = 19.9, P = 0.0008 | F(10,140) = 23.2, P < 0.0001 | F(10,140) = 6.05, P < 0.0001 | 5.6, 10, 18, 32, 56 mM |
| HCl | 2c | 9 WT, 10 KO (M) | F(1,17) = 353.6, P < 0.0001 | F(5,85)= 10.7, P < 0.0001 | F(5,85) = 1.16, P = 0.3360 | none |
| Citric acid | 1d | 11 WT, 11 KO (F) | F(1,19) = 4.33, P = 0.0512 | F(5,95) = 18.0, P < 0.0001 | F(5,95) = 0.63, P = 0.6744 | none |
| Denatonium | 1c, 7d | 8 WT, 9 KO(M) 11 WT, 10 KO (F) | F(1,36) = 14.6, P = 0.0005 | F(6,216) = 21.7, P < 0.0001 | F(6,216) = 3.98, P = 0.0009 | 0.032, 0.1, 0.32, 1 mM |
| QHCl | 2b | 9 WT, 10 KO (M) | F(1,17) = 34.6, P < 0.0001 | F(3,51) = 3.62, P = 0.0191 | F(3,51) = 1.33, P = 0.2760 | all* |
| Caffeine | 3c | 9 WT, 9 KO (M) | F(1,16) = 0.79, P = 0.3859 | F(4,64) = 27.0, P < 0.0001 | F(4,64) = 2.18, P = 0.0815 | none |
| ZnCl2 | 7c | 8 WT, 9 KO (F) | F(1,15) = 9.68, P = 0.0071 | F(4,60) = 49.8, P < 0.0001 | F(4,60) = 4.05, P = 0.0057 | 3.2, 10, 32 mM |
| Capsaicin | 2d | 9 WT, 10 KO (M) | F(1,17) = 5.77, P = 0.0280 | F(4,68) = 22.0, P < 0.0001 | F(4,68) = 0.80, P = 0.5280 | none |
| CaCl2 | 1a | 11 WT, 11 KO (F) | F(1,20) = 59.3, P < 0.0001 | F(4,80) = 18.6, P < 0.0001 | F(4,80) = 10.3, P < 0.0001 | 10, 32, 100 mM |
| CaLa | 2a | 9 WT, 10 KO (M) | F(1,17) = 53.6, P < 0.0002 | F(4,68) = 1.46, P = 0.2233 | F(4,68) = 3.81, P = 0.0075 | 1,3.2, 10, 32, 100 mM |
| MgCl2 | 3a, 8a | 18 WT, 18 KO (M) | F(1,34)= 23.2, P < 0.0001 | F(4,136) = 10.9, P < 0.0001 | F(4,136) = 1.27, P = 0.2861 | 10, 32 mM* |
| NaCl | 4a | 8 WT, 8 KO (F) | F(1,14) = 0.58, P = 0.4569 | F(5,70) = 22.8, P < 0.0001 | F(5,70) = 0.61, P = 0.6922 | none |
| NaLa | 5a | 9 WT, 8 KO (M) | F(1,15) = 0.70, P = 0.4150 | F(5,75) = 25.4, P < 0.0001 | F(5,75) = 1.20, P = 0.3162 | none |
| KCl | 4d, 5c | 9 WT, 8 KO (M) | F(1,31) = 7.95, P = 0.0083 | F(4,124) = 18.1, P < 0.0001 | F(4,124) = 3.03, P = 0.0201 | 100, 178 mM |
| 8 WT, 8 KO (F) | ||||||
| NH4Cl | 3d, 7b, 8b | 17 WT, 17 KO (M) | F(1,48) = 6.55, P = 0.0137 | F(5,240) = 26.9, P < 0.0001 | F(5,240) = 2.85, P = 0.0162 | 3.2, 32, 100 mM |
| 9 WT, 9 KO (F) | ||||||
Compounds are listed in the order presented in Figs. 3–5. Cohort and order provides batch of mice (1–8) and compounds tested in alphabetical order (a–d), with “a” being tested first, “b” second, and so on. Group composition gives number of wild-type (WT) and knockout (KO) mice tested; each cohort was either all M or all F. Concentrations supporting strain differences = P < 0.05 according to post hoc Fisher LSD tests.
*
Post hoc tests not strictly justified given the nonsignificant interaction (although a significant main effect of group). The analysis of KCl preferences omitted results of the test with 562 mM KCl because 18 of 33 mice spilled this concentration.