Table 3. Precision and accuracy of HPLC assay for paclitaxel from rat plasma and in cell line culture.

Spiked conc. (ng/mL)	Precision and accuracy data of paclitaxel (mean±SD) (%)
	Plasma				Cell Culture
	Measured conc.(ng/mL)	COV	%Deviation		Measured conc. (ng/mL)	COV	%Deviation
Intra-day (n = 3)
50	49.35±0.73	1.48	-1.36		48.24±0.52	1.07	-3.52
100	98.19±2.58	2.60	-1.81		106.21±1.98	1.86	+6.21
300	288.71±11.21	3.88	-3.74		284.16±8.91	3.13	-5.28
700	723.28±21.17	2.92	+3.32		671.65±19.65	2.92	-4.05
1,400	1356.11±39.2	2.89	-3.13		1313.92±45.52	3.46	-6.14
Inter day (n = 3)
50	48.95±0.94	1.92	-2.15		48.55±0.47	0.96	-2.91
100	97.12±2.27	2.33	-2.88		108.57±4.25	3.91	+8.57
300	282.71±10.11	3.57	-5.76		296.18±7.41	2.50	-1.27
700	634.28±46.59	7.36	-9.38		692.69±29.59	4.27	-1.04
1,400	1374.13±36.2	2.64	-1.84		1389.94±35.26	2.53	-0.71

Results are represented as mean±SD (n = 3). Accuracy and precision were determined with standard quality control samples, which were analyzed on 3 consecutive days. For intraday determinations, 3 standard curves were prepared on the same day, while for interday determinations, 3 standard curves were generated on three consecutive days. The accuracy is represented by %Deviation and precision by %COV.