Fig. 5. The specific CTL activity of mice triggered by various plasmid DNA vaccines.

Non-pulsed splenocytes (CFSE^low) and ESAT-6 peptides-pulsed splenocytes (CFS^Ehigh) from naive mice were transferred to the immunized mice. The representative histograms (A) and percentages (B) of splenic ESAT-6-specific lysis in the immunized mice were compared. **P < 0.01, vs. the PBS, pIRES or pIRES-FL group, respectively; ^ΔΔP < 0.01, meaning the DNA/Ags group vs. the corresponding DNA group, respectively. Images indicate that one representative result from three experiments, and data present as mean ± SEM (n = 10).