Figure 2. NCLX knockdown increases the Ca²⁺_mit in intact cells.

(a) Case12-mito (green) was localized in mitochondria (MitoTracker Orange, red). A merged image is shown on the bottom. (b) Case12-mito was co-transfected with control (control siRNA; white) or mNCLX siRNA (mNCLX siRNA; red) into HL-1 cardiomyocytes. The Ca²⁺_mit content, evaluated by normalizing the Case12-mito fluorescence of beating HL-1 cells to that of cells under a Ca²⁺_mit-chelated condition, was markedly decreased by knocking down NCLX (N = 15–16). * P < 0.05 vs control siRNA.