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Confirming detection of MAP DNA from mixed plaque samples.
Nested-PCR amplification of the 300 bp IS900 DNA region specific for MAP (Bull et al., 2003). Lane 1; DNA extracted from 5 M. smegmatis plaques. Lanes 2; DNA extracted from 5 MAP plaques. Lane 3; 4 MAP plaques mixed with 1 M. smegmatis plaque. Lane 4; 3 MAP plaques mixed with 2 M. smegmatis plaques. Lane 5; 2 MAP plaques mixed with 3 M. smegmatis plaques. Lane 6; 1 MAP plaque mixed with 4 M. smegmatis plaques.
