FIG. 5.

Immunofluorescence detection of MnSOD in MnSOD^tet/tet cells grown in the presence or absence of doxy. Immunolocalization of MnSOD in cell lines was carried out on coverslip preparations as described in the Materials and Methods. Each image pair shows MnSOD staining (green) on the left and MnSOD with actin (red) and nuclear staining DAPI (blue) on the right. MnSOD^tet/tet cells incubated in absence of doxy (panels A and B) or presence of doxy (0.25 μg/ml) doxy (panels C and D). MnSOD^+/+ cells incubated in absence of doxy (panels E and F) and presence of 0.25 μg/ml doxycycline (panels G and H). 60× magnification.