To determine if irradiation affected regulation of gene expression by miRNA, C57BL/6NTac mice were irradiated with 20 Gy to the pulmonary cavity. At various time points after irradiation, the mice were sacrificed, the lungs removed, mRNA was extracted, and RT-PCR was performed for: A: Tlr4, Tlr4-associated miRNA-107 and miRNA-511; B: Vegfa, Vegfa-associated miRNA-126; and C: Tgfb and Tgfb-associated mi-RNA-155.