Table II.
Effect of MMS350 treatment on modulation of endothelial cell gene transcription in irradiated lung.
| Gene | MMS350 Treatment | Days after 20 Gy | ||
|---|---|---|---|---|
| 28 | 150 | 200 | ||
| Vegf | No | 35.5 ± 3.5 | 112.4 ± 5.3 | 348.5 ± 67.2 |
| Yes | 24.3 ± 4.7 p = 0.1138 |
17.9 ± 3.6 p < 0.0001 |
262.0 ± 11.3 p = 0.2144 |
|
| Vwf | No | 82.0 ± 7.2 | 129.8 ± 8.9 | 85.0 ± 8.5 |
| Yes | 64.5 ± 9.5 p = 0.0257 |
87.6 ± 26.4 p = 0.0230 |
27.5 ± 4.9 p = 0.0143 |
|
| Nfkb | No | −84.5 ± 4.9 | 200.3 ± 30.3 | 202.5 ± 4.9 |
| Yes | −75.0 ± 1.4 p = 0.1208 |
128.8 ± 33.2 p = 0.0190 |
159.5 ± 4.9 p = 0.0130 |
|
| Nrf2 | No | −48.0 ± 8.0 | 96.0 ± 22.6 | 156.4 ± 8.0 |
| Yes | −71.7 ± 5.0 p = 0.0123 |
36.0 ± 11.3 p = 0.0786 |
139.6 ± 6.2 p = 0.1443 |
|
| Sod2 | No | 244.5 ± 7.8 | 140.1 ± 25.0 | 368.5 ± 6.4 |
| Yes | 123.5 ± 4.9 p = 0.0029 |
61.7 ± 23.7 p = 0.0039 |
255.0 ± 25.5 p = 0.0257 |
|
| Tgfb | No | 59.0 ± 1.4 | 255.2 ± 51.0 | 290.5 ± 29.0 |
| Yes | 41.5 ± 2.1 p = 0.0104 |
225.1 ± 10.3 p = 0.3732 |
180.5 ± 6.4 p = 0.0345 |
|
| Tlr4 | No | 4.0 ± 5.7 | 36.3 ± 7.8 | 329.5 ± 2.1 |
| Yes | 7.0 ± 1.3 p = 0.5475 |
18.7 ± 5.0 p = 0.0298 |
299.5 ± 3.5 p = 0.0093 |
|
| Igfbp7 | No | −7.3 ± 1.5 | 62.5 ± 3.5 | ND |
| Yes | −12.7 ± 1.2 p = 0.0474 |
38.2 ± 3.5 p = 0.0469 |
ND | |
C57BL/6NTac mice treated with MMS350 in the drinking water along with control mice were irradiated with 20 Gy to the pulmonary cavity and were sacrificed on day 28, 150 or 200 after irradiation. Lungs were removed, the endothelial cells isolated, mRNA was extracted, and RT-PCR was performed. Results represent percent induction of each gene transcript relative to baseline level. p-Values are for comparisons between mice treated with MMS350 and control irradiated mice. Significant p values are shown in bold. ND, not determined.