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. Author manuscript; available in PMC: 2013 Sep 26.
Published in final edited form as: Clin Cancer Res. 2012 Jul 2;18(18):4931–4941. doi: 10.1158/1078-0432.CCR-12-0697

Figure 5. Inhibition of oHSV efficacy by Copper and its rescue with ATN-224.

Figure 5

Glioma cells were infected with rQnestin34.5 (MOI=0.1) and incubated with buffer ± Copper (1 mg/L). A) Fluorescence microscopic images of GFP positive Gli36ΔEGFR glioma cells. B) Percent cell survival of U251T3, X12-V2, and Gli36ΔEGFR was measured 48 hrs post infection with rQnestin34.5 incubated with or without copper by standard crystal violet assay. *: P < 0.05 compared to rQnestin34.5. C–E): ATN-224 rescues copper-mediated inhibition of oHSV infection, replication and glioma cell killing. C) Fluorescent microscopy images of GFP positive Gli36ΔEGFR glioma cells infected with rQnestin34.5 incubated with Copper ± ATN-224. D) Viral titers were measured in cells infected with rQnestin34.5 pre-incubated with or without Copper ± ATN-224, 48 hrs post infection. *: P < 0.05 compared to rQnestin34.5 + Copper. E) Survival of cells infected with rQnestin34.5 pre-incubated with or without Copper ± ATN-224, 48 hrs post infection. *: P < 0.05 compared to rQnestin34.5 + Copper.