Skip to main content
. Author manuscript; available in PMC: 2014 Sep 1.
Published in final edited form as: Anal Biochem. 2013 Jun 5;440(1):71–77. doi: 10.1016/j.ab.2013.05.017

Figure 9.

Figure 9

Time-dependent inhibition by N-ethylmaleimide. AMSH (125 nM) was incubated with either 15 μM or 30 μM NEM for 1 h, 45 min, 30 min or 15 min followed by the addition of the K63POS1 probe (500 nM). Fluorescent measurements were taken following a 90 min incubation at 30°C. (n = 2)