Table 2.

Experimental data values for particle size and entrapment of albumin nanoparticles obtained by Taguchi orthogonal array table of L9 Design.

Albumin Concentration (% w/v)	Volume of BSA solution to total ethanol ratio (v/v)	Concentration diluted ethanol (% v/v)	Size (nm)	Entrapment efficiency (%)
3	1 : 0.75	100	20.01 ± 1.17	61.06 ± 1.94
3	1 : 0.90	70	15.72 ± 0.83	76.87 ± 2.03
3	1 : 1.05	40	24.69 ± 0.69	69.95 ± 3.84
4	1 : 0.75	70	20.14 ± 0.86	72.38 ± 2.42
4	1 : 0.90	40	25.65 ± 1.71	70.98 ± 1.06
4	1 : 1.05	100	88.90 ± 3.08	78.64 ± 1.97
5	1 : 0.75	40	58.17 ± 1.95	61.75 ± 0.84
5	1 : 0.90	100	191.53 ± 14.96	86.97 ± 2.71
5	1 : 1.05	70	130.69 ± 5.27	83.01 ± 2.09

Particle size and surface charge (zeta potential) were determined in PBS at 25°C by dynamic light scattering using a NICOMP ZLS 380 analyzer (PSS-NICOMP, Santa Barbara, CA, USA) [8]. Entrapment efficiency was determined by employing Vivaspin 500 ultracentrifuge filters with molecular cut-off weight of 300 K Da (Viva Products, Inc., Littleton, MA, USA). Aqueous filtrate generated at the bottom of Vivaspin 500 ultracentrifuge tubes was subjected to UV spectrophotometric analysis to determine the concentration of GEM (λ _max⁡ 248.6 nm; ε ₂₄₈ = 0.7053 × 10⁴ L mol⁻¹ cm⁻¹) [13].

Results are presented as mean ± SD, n = 3.