Figure 2.

Plasmacytoid dendritic cell (pDC) function in colonic tissue. (a) Percentages and (b) mean fluorescence intensities (MFIs) of colonic pDCs from naive and simian immunodeficiency virus (SIV)-infected macaques expressing intracellular interferon-α (IFN-α), macrophage inflammatory protein-1β (MIP-1β) and tumour necrosis factor-α (TNF-α) following imiquimod stimulation. Bars represent means ± SEM of six to eight animals per group. (c) Multi-parametric SPICE (version 5.22) analyses of the data shown in (a). Bars represent poly-functional subpopulations of stimulated pDCs and correspond to pie slices as shown. Arcs summarize overlap of each of the three functions. For multi-parametric analyses, non-responsive cells are not included in the analyses. Mann–Whitney U-tests were used for naive versus SIV comparisons; *P < 0·05. Only statistically significant differences are shown.