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. 2013 Sep 12;140(2):259–272. doi: 10.1111/imm.12136

Figure 6.

Figure 6

Effects of histone deacetylase inhibitors (HDACi) on CIITA and MHCII mRNA expression in DB diffuse large B-cell lymphoma (DLBCL) cells. (a) RNA was isolated from DB cells exposed for 0 and 24 hr to increasing doses of trichostatin A (TSA) and subjected to quantitative RT-PCR using primers for HLA-DRα, CIITA and GAPDH. Data are represented as the ratio of HLA-DRα or CIITA mRNA expression to GAPDH mRNA expression and are the average of at least three independent experiments. Standard errors are shown. (b) DB cells were transiently transfected with a luciferase gene vector containing 322 bp of the human CIITA pIII, cultured for 24 hr +/– 100 nm TSA, and subjected to luciferase assays. Data are represented as relative luciferase units (RLU) and are the average of four independent experiments. RNA was isolated from (c) DB and (d) NCI-H929 cells cultured for 24 hr with various concentrations of MS-275 and subjected to quantitative RT-PCR as described for Fig. 6(a). Cells treated with 100 nm TSA were included as a positive control for the effects of HDACi on CIITA and HLA-DRα mRNA expression. Data are the average of three independent experiments. Statistical significance is shown for each of the treatments (*P < 0·05; **P < 0·001; ***P < 0·0001; ns; not significant).