Table 2.

Effects of histone deacetylase inhibitor (HDACi) treatment on cell surface MHC expression

Cells/Treatment	Isotype	HLA-DR	HLA-ABC
Raji	3	487	601
RL	2	329	434
Daudi	4	452	6
Ramos	3	296	474
DB	3	2	6
100 nm trichostatin A	3	9	15
2 mm sodium butyrate	2	19	21
3 μm apicidin	4	8	12
5 mm valproic acid	2	8	n.d.
250 nm MS-275	3	71	28
SUDHL-6	4	108	97
100 nm trichostatin A	4	151	147
NCI-H929	3	2	81
50 nm trichostatin SA	4	2	110
2 mm sodium butyrate	3	2	203
100 nm MS-275	3	3	133

n.d., not done.

Cells were exposed for 24 hr to the HDACi shown and subsequently subjected to flow cytometry using antibodies to HLA-DR or HLA-A/-B/-C as described for Fig. 5. Values indicate the geometric mean fluorescence intensities and are representative of at least three independent experiments for each condition