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. 2013 Jun 24;591(Pt 18):4637–4653. doi: 10.1113/jphysiol.2013.255695

Figure 8. Luciferase reporter assay to test the relationship between miR-31 and HDAC4 and miR-31 and NRF1in C2C12 myoblasts.

Figure 8

N= 3 independent experiments with 3 replicates per experiment. pLuc2, empty luciferase control vector; p Luc2-HDAC4, luciferase vector contacting the HDAC4 3-UTR; p Luc2-NRF1, luciferase vector contacting the NRF1 3-UTR; mock, control miRNA.