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. 2013 Sep 8;174(1):27–37. doi: 10.1111/cei.12163

Fig. 4.

Fig. 4

BDC2·5 specificity and selection of donor-derived CD4+ T cells. (a) Frequency of host-derived (black bar) and donor-derived (white bar) cells in the thymus. The histograms show the mean frequency of host- and donor-CD4+ T cells ± standard error of the mean (s.e.m.) from five animals; ***P < 0·0005 (t-test). (b) Absolute numbers of donor-derived CD4SP thymocytes in the thymi of BDC2·5non-obese diabetic (NOD)→B6.g7 (n = 5) and BDC2·5NOD→B6.g7Rag–/– (n = 4) mice at 6 weeks post-haematopoietic cell transplantation (HCT). The histograms show the mean number of donor-CD4+ T cells ± s.e.m., *P < 0·01 (t-test). (c,d) Expression of T cell receptor Vβ4 on the donor-derived CD4+ T cells. Frequency of Vβ4 expressing cells in the donor-derived CD4+ CD25–forkhead box protein 3 (FoxP3)– cells in the thymus (c) and spleen (d) in the BDC2·5NOD→B6.g7 mice and BDC2·5NOD→B6.g7Rag–/– mice at week 6 post-transplant. (e,f) Percentage of donor-derived CD4+CD25-FoxP3– CD4+ T cells specific for BDC2·5 mimotope peptide, 2·5mi, was determined by flow cytometry staining of thymocytes (e) and splenocytes (f) with IAg7/2·5mi tetramer. Frequency of IAg7/2·5mi tetramer expressing cells is shown as mean ± standard error of the mean from four BDC2·5NOD→B6.g7Rag–/– and five BDC2·5NOD→B6.g7 mice. Data are pooled from two independent experiments.