Fig. 1.
Characterization and quantification of human granulocytic/neutrophilic myeloid-derived suppressor cells (Gr-MDSCs) in cord blood and different age groups. (a) Gr-MDSCs are characterized in the forward-/side-scatter (FSC/SCC) area as a granulocytic cell population in the peripheral blood mononuclear cells (PBMC) fraction (low-density neutrophils) (R1), as published previously [12,14,42]. This population was distinct from lymphocytes, monocytes, erythrocytes or debris. Representative scatter-plots are shown. (b) Within R1, Gr-MDSCs were identified as CD66bhighCD33highinterleukin IL-4Rαinterhuman leucocyte antigen D-related (HLA-DR)neg expressing neutrophilic MDSC population. Representative histograms are shown. (c) Log scale illustration of human Gr-MDSC numbers in cord blood and different age groups of healthy children and adults.