Skip to main content
. 2013 Sep 8;174(1):60–72. doi: 10.1111/cei.12157

Fig. 3.

Fig. 3

Human macrophages promote in-vitro wound repair through hepatocyte growth factor (HGF) secretion. (a) Representative images of Caco2 epithelial cell monolayers after wounding (day 0) or after 3 days of incubation. Caco2 cells were seeded with either Dulbecco's modified Eagle's medium (DMEM) 0·5% fetal calf serum (FCS) (–), or DMEM 10% FCS (+) or with 105 human macrophages (macrophages). (b) Wounded Caco2 cells were seeded in DMEM 0·5% FCS either directly with increasing numbers of macrophages, with 105 macrophages into a cell culture insert or with 105 peripheral blood mononuclear cells (PBMC). In negative (–) and positive (+) controls wounded Caco2 cells were seeded with DMEM 0·5% FCS or DMEM 10% FCS, respectively. Comparison between different numbers of macrophages was performed by a one-way analysis of variance (anova) test with Trend test as post-test. (c) c-Met inhibitor inhibits macrophage-assisted repair. Wounded Caco2 cells were seeded in DMEM 0·5% FCS with 300 pg/ml rHGF or with 105 macrophages in the presence or not of PHA-665732 (600 nM). (d) HGF protein concentrations measured by enzyme-linked immunosorbent assay (ELISA) in the culture supernatant of monocytes derived-macrophages or intestinal macrophages (IMACs). HGF concentrations were determined at 72 h of co-culture with wounded epithelial cells in DMEM 0·1% FCS. (e) Wounded Caco2 cells were seeded in DMEM 0·5% FCS with 105 macrophages isolated from human intestinal mucosa (IMACs). In negative (–) and positive (+) controls wounded Caco2 cells were seeded with DMEM 0·5% FCS or DMEM 10% FCS, respectively. (f) c-Met inhibitor inhibits IMACs-assisted repair. Wounded Caco2 cells were seeded in DMEM 0·5% FCS with 105 IMACs in the presence or not of PHA-665732 (600 nM). In negative (–) and positive (+) controls wounded Caco2 cells were seeded with DMEM 0·5% FCS or DMEM 10% FCS, respectively. Each dot represents a healthy donor. Comparison between several groups to identify a trend was performed by a one-way anova test with Trend test as post-test.