Fig. 1.

Gating strategy for analysis of CD146 expression on T cells. Peripheral blood mononuclear cells (PBMCs) were gated sequentially to exclude doublets and include lymphocytes. T cells were then gated as CD3⁺ T cells, and usually (except in Fig. 3) divided further into CD4⁺ and CD4⁻ T cell subsets (the latter representing mainly CD8⁺ T cells). Both subsets were then analysed for expression of CD146 and an additional marker (CD25 is shown here). The cut-off for positive staining was established using isotype controls (bottom right).