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. Author manuscript; available in PMC: 2014 Oct 1.
Published in final edited form as: Neuropharmacology. 2013 May 17;73:19–30. doi: 10.1016/j.neuropharm.2013.05.001

Figure 5.

Figure 5

Neuronal activation by DHβE in the dorsal ST and SNr. WT and homozygous Leu9’Ala were perfused 150 minutes after a challenge of saline (i.p.) or DHβE (1 mg/kg, i.p.) and coronal sections (20 µm thick) from the dorsal ST or SNr were isolated and immunolabeled to detect c-Fos and ChAT expression (ST) or TH expression (SNr). a) Top, representative brain atlas picture of illustrating ST region analyzed. An immuno-labeled coronal section from Leu9’Ala mice after injection with DHβE depicting c-Fos (red) and ChAT (green) expression is shown. DAPI stained nuclei are labeled blue. Bottom, number of c-Fos immuno-positive neurons/slice in WT and Leu9’Ala mice after saline or DHβE injection (3 mice/ treatment, 10 slices/mouse). b) Top, representative brain atlas picture of illustrating SNr region analyzed. An immunolabeled coronal section from Leu9’Ala mice after injection with DHβE depicting c-Fos (green) and TH (red) expression is shown. DAPI stained nuclei are labeled blue, c) Bottom, quantification of c-Fos immuno-positive neurons in WT and Leu9’Ala after saline or DHβE (3 mice/ treatment, 10 slices/mouse). ***p<0.001