Figure 4.

Overexpression of platelet derived growth factor B or transforming growth factor β₁ inhibits the adipogenic differentiation of mesenchymal stem cells/bone marrow stromal cells (MSCs). Transduced MSCs were cultured in adipogenic medium for 21 days. (A): Cells stained with Oil-Red O and pictured in representative areas. Scale bar = 100 mm. (B): Number of adipocytes counted microscopically (n = 4). (C): Quantification of cells with high triglyceride, by means of Nile Red stained cells, using flow cytometry (n = 3). (D, E): Quantification after 14 days under adipogenic media of mRNA levels of adipogenic markers ppar-g and fabp4, respectively (n = 4). Abbreviations: bFGF, basic fibroblast growth factor; fabp4, fatty acid binding protein 4; PDGF-B, platelet derived growth factor B; ppar, peroxisome proliferator-activated receptor γ; TGF-β1, transforming growth factor β₁; VEGF, vascular endothelial growth factor.