Figure 10.

Spastizin depletion induces accumulation of autophagosomes in primary hippocampal neurons. Primary hippocampal neurons were transfected with MAP1LC3B-RFP for the staining of autophagosomal vesicles (red) and with a control vector (ctr) or with two specific Zfyve26 (Spg15) short hairpin RNAs (shRNA1 and shRNA2) to silence the gene, all with a GFP tag. Seventy-two hours later neurons were starved with Dulbecco’s modified Eagle’s medium for 4 h and fixed. LC3-positive vesicles quantification is shown in the graph. The graphs show the mean ± SEM of three independent experiments for a total of at least 30 cells for each sample (***P < 0.001). Scale bar = 10 µm.