Figure 6.

Spastizin mutations induce accumulation of autophagosomes in fibroblast cells. Control (ctr) and L243P, R1209fsX and S1312X spastizin mutated fibroblast cells were transfected with MAP1LC3B-RFP for the staining of autophagosomal vesicles (red) and 24 h later were starved for 2 h to induce autophagy (starvation), fixed and immunostained with anti-p62 (green) antibody. Yellow in the merge images indicates co-localization of p62 and LC3. LC3-positive vesicles quantification is shown in Fig. 7B. Scale bar = 10 µm. unt = untreated.