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Characterization of LT-specific CD4+ T cells using splenocytes stimulated with LT peptide #29. (A) Schematic regimen of vaccination schedule used for in vivo tumor protection experiments. (B) Intracellular cytokine staining using phycoerythrin (CD4 labeling) and fluorescein isothiocyanate (IFN-γ labeling) as fluorescent tracers followed by flow cytometry analysis to characterize LT-specific CD4+ T cell responses using splenocytes stimulated with candidate peptide #29.
