TRPS-1 expression is positively correlated with E-cadherin (E-cad). (A) Examples of two cases by IHC, one with low TRPS-1 (QS < 4) and E-cad (1+) levels, and one with higher TRPS-1 (QS ≥ 4) and high E-cad (3+) levels. (B) Table summarizing analysis of 79 ER+ BC cases showing a significant association between TRPS-1 and E-cadherin expression (P < 0.001). (C) Co-staining and immunofluorescence microscopy of TRPS-1 with E-cadherin, TRPS-1 with vimentin in T47D, BT474, and SUM159 BC cell lines. Most T47D and BT474 cells co-expressed TRPS-1 and E-cadherin, and did not express vimentin, a mesenchymal marker, while a few cells which did not express TRPS-1, also did not express E-cadherin (yellow arrows in merged images). In contrast, SUM159 over-expressed vimentin, but did not express TRPS-1 and E- cadherin.