Liver samples were embedded in Tissue-Tek optimal cutting temperature compound (Sakura Finetek), frozen, and sectioned (7 μm), then were stained Oil Red O and hematoxylin. Sections were photographed at ×400 magnification. Panel A, liver from control mice on chow; Panel B, liver from SMS2LTg mice on chow. Panel C-J, All the mice were on a high fat high cholesterol diet for 8 weeks. Panel C-D, liver, from control and SMS2LTg mice, size comparison. Panel E-F, liver, from control and SMS2 KO mice, size comparison. Panel G, Control liver. Panel H, SMS2LTg mouse liver. Panel I, Control liver. Panel J, SMS2 KO mouse liver. This set of result is the representatives of 6 WT and 6 SMS2LTg mice, and 6 WT and 6 SMS2 KO mice. *P<0.05.