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. Author manuscript; available in PMC: 2014 Sep 1.
Published in final edited form as: Cancer Chemother Pharmacol. 2013 Jul 24;72(3):597–606. doi: 10.1007/s00280-013-2231-9

Fig. 1. Initial rates and kinetic analysis of [3H]pralatrexate and [3H[MTX uptake.

Fig. 1

The time-course of drug uptake over 15 min at an extracellular concentration of 0.05 M (Panel a) or over 80 sec at an extracellular concentration of 0.5 M (Panel b) at pH 7.4. The data are the mean ± SEM from three independent experiments. Panel c: [3H]pralatrexate influx as a function of substrate concentration assessed over 1 min at pH 7.4 in presence or absence of 5 M non-labeled MTX. The lines are best-fit to the Michaelis-Menten equation (V = Vmax[S]/(Kt + [S])). Panel d: A double-reciprocal plot of the data (Lineweaver-Burk). Results are the mean ± SEM from four independent experiments.