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. Author manuscript; available in PMC: 2014 Oct 1.
Published in final edited form as: Hear Res. 2013 Aug 2;304:167–178. doi: 10.1016/j.heares.2013.07.017

Figure 5.

Figure 5

The core subunit of the NuRD repressive complex, CHD4, is detected in a similar pattern of expression as HDAC1, HDAC2, and KDM1A. Immunofluorescence of wild type organ of Corti was performed using antibodies against CHD4 (red), PVALB (green), and Hoechst (blue). Taqman gene expression assays were done on microdissected organ of Corti. Expression levels are relative to endogenous gene controls 18S, GAPDH, and Actb. Fold changes are shown relative to P0. (A & A’) CHD4 is highly present throughout the organ of Corti at E18.5. (B-C’) From P0 to P2, CHD4 continues to be detected in the vast majority of cells in the organ of Corti. (D & D’) While at P4, CHD4 continues to be detected throughout the organ of Corti but appears reduced in intensity. (E & F’) By P6, CHD4 staining is only found in the IHCs of the organ of Corti, and is no longer apparent by P7. (G-H’) KDM1A is detected again in the organ of Corti by P8 and continues to be present through P21. (I) Similar to Hdac1, Hdac2, and Kdm1a, Chd4 mRNA levels steadily decrease in expression from P0 to P6, and then at P7 and P21 expression is increased compared to the P0 time point.. All representative images were taken from the middle turn of the cochlea. *P<0.05 by Data Assist Software.