FIGURE 1:

Deletion of individual polarisome component genes (except msb3 and msb4) significantly suppresses the cER inheritance defects in ptc1∆ mutant cells. (A) Representative GFP fluorescence of ER marker Hmg1p and differential interference contrast (DIC) images of wt, bud6∆, bni1∆, ptc1∆, bud6∆ptc1∆, and bni1∆ptc1∆ cells grown to early log phase in SC medium at 25°C (sph1∆, sph1∆ptc1∆, pea2∆, pea2∆ptc1∆, spa2∆, spa2∆ptc1, msb3∆, msb3∆ptc1, msb4∆, and msb4∆ptc1 images are shown in Supplemental Figure S2). Arrowheads point to small buds. Bar, 5 μm. (B) The percentage of small buds that contained normal cortical ER in indicated strains was quantified. Error bars, SEM from three independent experiments.