FIGURE 2:

Deletion of PEA2 or SPA2 affects Slt2-GFP localization at bud tip or bud neck, whereas the deletion of other polarisome component genes does not. (A) Slt2-GFP fluorescence and DIC images of representative cells grown to early log phase in SC medium at 25°C. The strains shown are wt, ptc1∆, bud6∆, bni1∆, pea2∆, sph1∆, spa2∆, msb3∆, msb4∆, or msb3∆msb4∆ double mutant containing a plasmid expressing an Slt2-GFP fusion from the endogenous promoter (ptc1∆, sph1∆, spa2∆, msb3∆, msb4∆, and msb3∆msb4∆ images are shown in Supplemental Figure S3). Arrowheads and arrows point to the bud tip in small buds and bud neck in large buds, respectively. Bars, 5 μm. (B) The Slt2-GFP localization in small buds from the strains examined was quantified. Error bars in the bar graph are SEM from three independent experiments.