FIGURE 4:

The effects of latrunculin A (Lat-A) treatment on cER inheritance in wild-type or mutant cells (ptc1∆, sec3∆ or myo4∆). Wild-type or mutant yeast cells grown to early log phase in SC medium at 25°C were incubated with control dimethyl sulfoxide or 200 μM Lat-A for 10 min. Cells were immediately fixed, washed, and then imaged with the ER marker Hmg1p-GFP. (A) Representative cell staining. Arrowheads point to small buds. (B) Percentage of cells with small buds containing normal cER staining with or without Lat-A treatment. Error bars in the bar graph represent SEM from three independent experiments.