Figure 4. LDH-A is essential for network formation on Matrigel in vitro.

3x10⁴ cells from Tet-On and Tet-Off systems were seeded on Matrigel in 48 wells plate and kept at 37°C overnight. Cells expressing high LDH-A levels from both systems started to form networks as early as two hours after seeding, and complete networks were observed after 24 hours. Cells with low LDH-A levels failed to form networks.