|
WUS-F |
5'-GCTAATTCCGTCAACGTTAAAC-3’
|
|
WUS-R |
5'-TTTAAATTCCCGTTATTGAAGC-3’
|
|
WEE1-F |
5’-TTGGACAAAAGCTTACCAGTAGAAG-3’
|
|
WEE1-R |
5’-AGAGAAGATATCGACTTTATCAAGG-3’
|
|
HIS4-F |
5’-TTAGGCAAAGGAGGAGCAAA-3’
|
|
HIS4-R |
5’-CTCCTCGCATGCTCAGTGTA-3’
|
|
CycD3;1-F |
5’-GCAAGTTGATCCCTTTGACC-3’
|
|
CycD3;1-R |
5’-CAGCTTGGACTGTTCAACGA-3’
|
|
CycB1;1-F |
5’-CTCAAAATCCCACGCTTCTTGTGG-3’
|
|
CycB1;1-R |
5’-CACGTCTACTACCTTTGGTTTCCC-3’
|
|
CycA1;1-F |
5’-GGCTAAGAAGCGACCTGATG-3’
|
|
CycA1;1-R |
5’-TACAAGCCACACCAAGCAAC-3’
|
|
CycB2;3-F |
5’-TAAACCACCTGTGCATCGAC-3’
|
|
CycB2;3-R |
5’-ATCTCCTCCAGCATTGCTTC-3’
|
|
E2Fa-F |
5’-ACGCTGGTTCTCCTATCACAC-3’
|
|
E2Fa-R |
5’-GGCTTGTTTAATTAGATTGACGAA-3’
|
|
E2Fc-F |
5’-GGAAGGGTGCTGACAATCTT-3’
|
| E2Fc-R |
5’-CATCCAACCTGCTTTCCTCA-3’
|
|
DPa-F |
5’-GATGATTCTGAAATTGGATCAGAG-3’
|
|
DPa-R |
5’-TTGGCTTCCAACTTCTGACA-3’
|
|
GRP23-F |
5’-TGCTCCATCCTCAGTTACTT-3’
|
|
GRP23-R |
5’-AATAAACTCGCAGCATCTCC-3’
|
|
ACTIN-F |
5’-GCTCCTCTTAACCCAAAGGC-3’
|
|
ACTIN-R |
5’-CACACCATCACCAGAATCCAGC-3’
|
|
ARR5-F |
5'-TTTGCGTCCCGAGATGTTAG-3’
|
|
ARR5-R |
5'-CCATACTATCATCAACAGCAAGAAC-3’
|
|
ARR6-F |
5’-TTGCCTCGTATTGATAGATGTC-3’
|
|
ARR6-R |
5’-CGAGTGAACAGGGTAGACATT-3’
|
|
ARR7-F |
5’-AATGCCAGGACTTTCAGGAT-3’
|
|
ARR7-R |
5’-ATTCCTCTGCTCCTTCTTTG-3’
|
|
RT-PCR
|
|
|
WUS-pBI221-F |
5'-CCGCTCGAGATGGAGCCGCCACAGCATCAGCATC-3’
|
|
WUS-pBI221-R |
5'-GGGGTACCCTAGTTCAGACGTAGCTCAAGAGAA-3’
|
|
GRP23-LUC-F |
5’-CGGGATCCTATCCAGCTAATCCCATCTGCTCTT-3’
|
|
GRP23-LUC-R |
5’-CCCAAGCTTGGTGGAGGGAAAATGATTTAGGGTT-3’
|
|
CLV3-LUC-F |
5’-CGGGATCCGCAACCTTCGATAGAAATAGTGAC-3’
|
|
CLV3-LUC-R |
5’-CCCAAGCTTAAGACACAAGTATATCTCCAAAGC-3’
|
|
GRP23 RNAi-F |
5’-GGCGCGCCGGATCCCGGAGATGCTGCGAGTT-3’
|
|
GRP23 RNAi-R |
5’-CATGCCATGGTCTAGACTTACTTCCGACCTTCTT-3’
|
|
ACTIN-F |
5’-TTTGCGACAATGGAACTG-3’
|
|
ACTIN-R |
5’-AAGAGCAATGTAGCAAAG-3’’ |
|
EMSA
|
|
|
GRP23p-Probe-F |
5'-CATATATCTTTAATACTGTTAATGATCTTTCTTCAAAAAC-3’
|
|
GRP23p-Probe-R |
5'-GTTTTTGAAGAAAGATCATTAACAGTATTAAAGATATATG-3’
|
|
ChIP
|
|
|
GRP23p i-F |
5'-TCACGTTATATGAGCATCTTTT-3’
|
|
GRP23p i-R |
5'-TTGAAACTGA AACTTTATACGAAA-3’
|
|
GRP23p ii-F |
5'-ACCAGCTATGGATTATTTGAGA-3’
|
|
GRP23p ii-R |
5'-AAGACAAGTAAAGAAAGGTTGG-3’
|
|
GRP23p iii-F |
5'-CGTATTACCAAACAGCCCTC-3’
|
|
GRP23p iii-R |
5'-CCTTGGATGTGAAGAAATGG-3’
|
|
UBQ-F |
5'-CAGGATAAGGAGGGCATT-3’
|
|
UBQ-R |
5'-TTTCCCAGTCAACGTCTT-3’
|
