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. Author manuscript; available in PMC: 2013 Sep 26.
Published in final edited form as: Methods Mol Biol. 2013;1046:359–370. doi: 10.1007/978-1-62703-538-5_22

Fig. 1.

Fig. 1

Mounting the imaging chamber. (a) Front view of the metal chamber and the coverslip. (b) Add grease to the inner part of the metal chamber (1) and place the coverslip on top of the greased area with the cell facing the grease (2). (c) Cover the coverslips with media. (d) Example of EGF stimulation. Approximately 800 μL can be added to the imaging chamber without spilling the imaging solution