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. Author manuscript; available in PMC: 2014 Jul 5.
Published in final edited form as: Chem Biol Interact. 2013 May 14;204(2):116–124. doi: 10.1016/j.cbi.2013.04.016

Figure 1. Time course of MG132 induced cytotoxicity.

Figure 1

The induction of apoptosis and necrosis was measured in N27 cells at 6 and 24h following treatment with MG132 (1μM). (A) The representative immunoblot shows caspase 3 cleavage increased to a significant level at 24h. (B) Fold changes of cleaved caspase 3 are normalized by control and estimated by densitometry. (C) Apoptosis was also measured in N27 cells using annexin V/PI cell staining in combination with flow cytometry. Significant apoptosis and necrosis were observed after 24h treatment. These data are presented as mean ± SD, (n=3–6); **p<0.01 and ***p<0.001 are considered significant by ANOVA using Dunnet’s multiple comparison test.