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. 2013 Sep 26;8(9):e76637. doi: 10.1371/journal.pone.0076637

Figure 3. Evaluation of the Tal2 transcriptional start site in human and mouse.

Figure 3

(A) 5’-RACE (rapid amplification of cDNA ends) defines transcription start sites of Tal2 in distinct cells. 5’-RACE was performed using mRNA from murine RAW, U937 and K562 cells of human origin. Upon PCR the products were analysed on a gel, sequenced and aligned to the genome. Two different start sites were defined and named the P1-promoter and the P2-promoter. Specific products are marked with an arrow. (B) Schematic representation of the human and mouse genomic region of Tal2. The human Tal2 gene has two alternative promoters hP1 and hP2. hP1 is located at about -6500 bp from the ATG and includes an alternative non-coding exon. In mouse RAW cells the mP1 promoter is active. The position of primer pairs for ChIP is marked. (C) In a luciferase experiment the promoter activity of the first 500 bp of the human hP1 and hP2 region was compared with the mouse mP1 and mP2 region in murine RAW cells. (D) Luciferase experiment comparing the promoter activity of the first 500 bp of the human hP1 and hP2 region with the mouse mP1 and mP2 region in human K562 cells. Values are given as fold related to the luciferase values gathered with the empty pGL4 vector. Error bars give the standard deviation of four determinations.