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. 2013 Sep 26;8(9):e76637. doi: 10.1371/journal.pone.0076637

Figure 5. The human Tal2 promoter is activated by GATA1 and PU.1. (A).

Figure 5

The sequence of the human Tal2 P2-promoter is shown and identified potential transcription factor binding sites are marked in colour. (B) PU.1 and GATA1 activated the hTal2(-495/+16) promoter in HEK293 cells. (C) Mutation of the PU.1 and GATA1 site, respectively, diminish activation by the transcription factor. Wild type or mutant promoter construct were cotransfected with the given transcription factor and luciferase activity measured after 48 hours. Error bars give the standard deviation of at least six evaluations. The mutated sites are underlined.