Table 1. Predicted antigenic peptides within the Glossina morsitans morsitans Ada, Tsgf1 and Ag5 salivary proteins.

Consensus peptides	E-value G.m. morsitans	Best match specie (E-value)
ADA
Seq171–96:	2.10−16	Burkholderia pseudomallei (0,96)
HLSTGGEVQLNSKEIEVDRIFMKYKI
Seq2110–135	6.10−17	Drosophila mojavensis (5.10−13)
AAGMHFFKAKPLIERSKVFRFLQQMP
Seq3168–193	6.10−17	Leptospirillum ferrodiazotrophum (1,7)
DQRGINILTFRENPERHKCTTQYVAV
Seq4174–199	4.10−17	Drosophila mojavensis (0,0088)
ILTFRENPERHKCTTQYVAVNEERQK
Seq5188–213	3.10−16	Drosophila melanogaster (5,6)
TQYVAVNEERQKSRSQADYNRSFENL
Seq6292–317	1.10−15	Drosophila pseudoobscura pseudoobscura (4,2)
DVLTVILEVVGSFRSQYPDFLGVKLI
Seq7315–340	1.10−16	Arthrospira platensis str. Paraca (2,3)
KLIYAINRRLETNEVRNRVEILKKFQ
TSGF1
Seq11–20	1.10–10	Methylobacter tundripaludum (0,17)
EVNKAYQDERNKILQEEEKL
Seq218–43	4.10–15	Marinobacter algicola (1,3)
EKLRLGYDVILQGDEEKADQIFLRLK
Seq3130–156	3.10–16	Paramecium tetraurelia (0,4)
DGCKSESVQVSKGEAKEDWFNLYTPT
Seq4207–232	3.10–16	Candida tropicalis (0,71)
EVRVKFSEPYDDTSKKYSLDDVVKEI
Seq5436–461	9.10–15	Lactobacillus delbrueckii subsp. Bulgaricus (0,4)
VENTIKYAQLNSQEKTAETLLKAKWN
AG5
Seq114–39	2.10−14	Daphnia pulex (3)
VACVSQNVFQSGCSSDAKMIDLKKYQ
Sep2105–130	2.10−16	Vibrio mimicus (7,8)
NLAELGRSGAGTPDYGQLIQKAVDS
Seq3203–226	2.10−13	Drosophila melanogaster (1,7)
KSCSASAQDCKTGKNSKYQNLCSA
Seq4221–243	1.10−14	Stomoxys calcitrans (0,008)
QNLCSANEKYEVNRWFKDGVEYQ

All peptides that were identified by at least three out of four epitope prediction servers (NETSurfP, ABCpred, Bcepred and Proped-2) are listed for each protein. Within peptide sequences, amino acids forming the linear epitope are bolded. Each peptide was then blasted on all non-redundant Genbank CDS databases. Blast E-value for G. morsitans morsitans and the best match species are indicated. For each protein, the candidate peptide selected for biological validation is underlined.