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. 2013 Sep 26;9(9):e1003749. doi: 10.1371/journal.pgen.1003749

Figure 4. S613 is conserved throughout evolution.

Figure 4

PER protein sequence alignment with various (A) Drosophila, (B) insect, and (C) vertebrate species. Alignments were generated using the Clustal-W Method within the MegAlign program contained in the DNA Star software package. Alignment Reports were exported and cropped to highlight the indicated residue stretches. Note the evolutionary conservation of the S613 (second yellow box) and Y614 (green box) residues; prolines are highlighted in a blue box. (D) Graphs depicting period length of a wild type transgenic fly line (brown bar) or those harboring the P611A (dark blue bars), P612A (light blue bars), or Y614A (green bars) mutations. Data from all transgenic lines are shown. Period lengths are listed within each bar and values in parentheses indicate the number of flies analyzed. Raw data are summarized in Table 1. Only male flies were analyzed in these experiments.