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. 2013 Sep 26;9(9):e1003654. doi: 10.1371/journal.ppat.1003654

Figure 5. Concentration-dependent inhibition of QS-regulated phenotypes in Gram-negative bacteria.

Figure 5

(A) Prodigiosin production in S. marcescens. Cells were grown in MB medium with serial dilutions of yayurea A or B at 28°C. Relative prodigiosin production was calculated as the ratio between prodigiosin content (absorbance at 534 nm) and cell density (absorbance at 600 nm). (B) Bioluminescence in V. harveyi. Cells were grown in marine broth with serial dilutions of the compounds at 28°C for 24 h. Relative luminescence units were normalized by the cell density. (C) Biofilm formation of P. aeruginosa. Cells were grown in LB with serial dilutions of yayurea A or B at 37°C for 24 h. (D) Biofilm formation of S. aureus. Cells were grown in TSB with serial dilutions of yayurea A, B, or gallidermin (positive control) at 37°C for 24 h. Biofilm cell layer was visualized by crystal violet staining and measured at 590 nm. Microtiter plates presented are representative of at least three independent sets of experiments. Bars indicate standard deviation of the mean, SD.