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. 2013 Sep 26;9(9):e1003809. doi: 10.1371/journal.pgen.1003809

Figure 6. Western immunoblotting analysis of B4GALNT2 transferase activity in Lacaune sheep granulosa cells and follicular fluids.

Figure 6

Granulosa cell protein extracts (50 µg) and follicular fluids (200 µg) from +/+ and L/L large antral follicles were precipitated (P) by agarose-DBA lectin or sepharose-protein A-KM694 monoclonal antibody. The resulting purified glycoproteins were separated on SDS-PAGE, transferred on nitrocellulose membrane and revealed after blotting (B) using biotinylated-DBA lectin or KM694 monoclonal antibody.