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. 2013 Sep 26;9(9):e1003831. doi: 10.1371/journal.pgen.1003831

Figure 3. Constitutively active PKA in GABAergic neurons partially bypasses the requirement of AEX-2/GPCR.

Figure 3

(A) Diagram showing the construction of the constitutively active PKA (PKA[CA]). “R” and “C” indicate the PKA regulatory and catalytic subunit, respectively. The two asterisks (*) represent the two mutations (H96Q, W205R) in the PKA catalytic subunit KIN-1a, which presumably disrupt its association with the regulatory subunit but do not affect its enzymatic activity. (B) Quantification of the Exp step of young adults with the indicated genotypes. PKA[CA] denotes PKA constitutively active transgenic worms (vjIs102 and vjIs103) expressing the mutated catalytic subunit kin-1a(H96Q, W205R) in the GABAergic neurons using the unc-47 full length promoter. vjIs103 and vjIs77 were used for the PKA[CA];PKA[DN] strain. (C) Representative ethograms of ten consecutive defecation cycles of young adult worms with the indicated genotypes. Each dot represents 1 s. “p” stands for the pBoc step and “x” indicates the Exp step. Ectopic Exp steps are indicated by “x”. Means and standard errors are shown. Asterisks indicate significant differences between indicated groups: ** P<0.01, *** P<0.005 in Student's t-test.