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. 2013 Sep 26;9(9):e1003831. doi: 10.1371/journal.pgen.1003831

Figure 6. unc-2/VGCC functions downstream of PKA to mediate calcium influx in the GABAergic neurons.

Figure 6

(A) Quantification of the Exp step of young adult worms with the indicated genotypes. vjIs103 was used for the PKA[CA] strain. (B) and (C) Representative trace of GCaMP3 fluorescence in the synaptic region of DVB neurons in unc-2 mutants in normal cycles, with the Exp step (B) and incomplete cycles, without the Exp step (C). (D) Comparison of the maximal change (ΔFmax/F0) of GCaMP3 fluorescence in the synaptic region of DVB neurons between the normal cycles and the incomplete cycles in unc-2 mutants and unc-25 mutants. vjIs58, the transgenic strain with GCaMP3 expressed in the DVB neuron, was used. Normal cycles: n = 22 in unc-2(lj1) mutants; n = 10 in unc-25(e156) mutants. Incomplete cycles: n = 18 in unc-2(lj1) mutants; n = 31 in unc-25(e156) mutants. Means and standard errors are shown. Asterisks indicate significant differences: * P<0.05, *** P<0.0005 in Student's t-test.