Skip to main content
. 2013 Sep 26;9(9):e1003831. doi: 10.1371/journal.pgen.1003831

Figure 7. Other non voltage-gated calcium channels are required from calcium influx in the GABAergic neurons.

Figure 7

(A) and (B) Quantification of the Exp step of young adult worms with the indicated genotype types. (C) Classification of different patterns of pBoc, fluorescent spikes in DVB and Exp in worms with the indicated genotypes. unc-2: 40 cycles in 21 animals, egl-19; unc-2: 19 cycles in 11 animals, PKA[CA]; egl-19; unc-2: 24 cycles in 12 animals, PKA[CA]: 23 cycles in 12 animals. (D) and (E) Quantification of the duration and amplitude of regular DVB calcium spikes in worms with indicated genotypes. PKA[CA] represents transgenic worms with constitutively active PKA specifically expressed in GABAergic neurons (vjIs103 in (B) and vjIs102 in (C, D)). Note that in (C), (D) and (E), unc-2 and PKA[CA] strains, but not the egl-19; unc-2 and the PKA[CA]; egl-19; unc-2 strains contain the unc-13(s69) mutation for immobilization, as egl-19;unc-2 alone were almost completely paralyzed. vjIs58, the transgenic strain with GCaMP3 expressed in the DVB neuron, was used for the unc-2 strain; while vjIs64 was used for calcium imaging in other genotypes. Means and standard errors are shown. Asterisks indicate significant difference between indicated group and significant difference from wild type in (A), PKA[CA] in (B) and egl-19; unc-2 mutants in (D) and (E): * p<0.05; ***P<0.005 in Student's t-test. “n.s.” indicates no significant difference between indicated groups.