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. 2013 Sep 24;123(10):4195–4207. doi: 10.1172/JCI62891

Figure 5. Overexpression of SALL4 induces enrichment of SALL4 binding and epigenetic activation markers at the HOXA9 promoter region and increases HOXA9 expression.

Figure 5

(A) Human HOXA9 promoter region. The locations of the primers used in ChIP-qPCR (arrows) are relative to the translation start ATG. (B) ChIP-qPCR demonstrated that SALL4 binding at HOXA9-I was enriched by overexpression of SALL4B in KG1a cells compared with cells treated with control vector (pMig). In contrast, HOXA9-III showed no enrichment of SALL4 binding. ChIP was performed using SALL4 antibody with KG1a cells transduced with control pMig-GFP or pMig-GFP-SALL4B vectors. GFP+ cells were used in this experiment. All values are averages calculated from 3 separate SALL4 pulldowns. (C) Enrichment of epigenetic markers in the HOXA9 promoter region induced by SALL4B overexpression. ChIP was performed using H3K4, H3K79, or POLII antibody on KG1a cells treated as in B. (D) qRT-PCR analysis of SALL4B and HOXA9 expression in pMig-SALL4B– and control vector–transduced GFP+ KG1a cells. Data in B–D are mean ± SD from 3 independent experiments. *P < 0.05, **P < 0.001.